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BMED13-214: Molecular Diagnostics May 2019 [Standard]

General information

In this subject, students will learn about the regulation of gene expression, the human genome and the techniques that are available to study genes. Students will be introduced to the tools of bioinformatics and use these to analyse human genes and genetic diseases. Advanced studies in the molecular biological techniques used to investigate patterns of gene expression will be an important part of this subject. This subject will interest students who wish to undertake a career in a research or a biomedical laboratory setting.


Academic unit:Faculty of Health Sciences and Medicine
Subject code:BMED13-214
Subject title:Molecular Diagnostics
Subject level:Undergraduate
Semester/Year:May 2019
Credit points:10

Delivery & attendance

Delivery mode:


Workload items:
  • Science Lab: x8 (Total hours: 24) - Science Lab
  • Lecture: x12 (Total hours: 24) - Weekly Lecture
  • Tutorial: x12 (Total hours: 12) - Weekly Tutorial
  • Personal Study Hours: x12 (Total hours: 60) - Personal Study
Attendance and learning activities: Attendance is compulsory Case 1: DNA extraction, PCR-RFLP, electrophoresis, DNA sequencing Case 2: RNA extraction, cDNA synthesis, RT-qPCR, FISH


Prescribed resources:
  • Michael R. Cummings, Michael A. Palladino, William S. Klug, Charlotte A. Spencer (2015). Concepts of Genetics. 11th, Pearson , 896. 26
  • Lela Buckingham (2012). Molecular Diagnostics: Fundamentals, Methods and Clinical Applications. 2nd, Philadelphia: F.A. Davis Company , 558. 16
After enrolment, students can check the Books and Tools area in iLearn for the full Resource List.
[email protected] & Email:[email protected] is the online learning environment at Bond University and is used to provide access to subject materials, lecture recordings and detailed subject information regarding the subject curriculum, assessment and timing. Both iLearn and the Student Email facility are used to provide important subject notifications. Additionally, official correspondence from the University will be forwarded to students’ Bond email account and must be monitored by the student.

To access these services, log on to the Student Portal from the Bond University website as

Enrolment requirements

Requisites: ?


Restrictions: ?


Assurance of learning

Assurance of Learning means that universities take responsibility for creating, monitoring and updating curriculum, teaching and assessment so that students graduate with the knowledge, skills and attributes they need for employability and/or further study.

At Bond University, we carefully develop subject and program outcomes to ensure that student learning in each subject contributes to the whole student experience. Students are encouraged to carefully read and consider subject and program outcomes as combined elements.

Program Learning Outcomes (PLOs)

Program Learning Outcomes provide a broad and measurable set of standards that incorporate a range of knowledge and skills that will be achieved on completion of the program. If you are undertaking this subject as part of a degree program, you should refer to the relevant degree program outcomes and graduate attributes as they relate to this subject.

Find your program

Subject Learning Outcomes (SLOs)

On successful completion of this subject the learner will be able to:
  1. Demonstrate knowledge of the techniques that molecular biologists use to manipulate and analyse nucleic acids.
  2. Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
  3. Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing,  RT-qPCR and FISH
  4. Demonstrate understanding of the Human Genome Project and associated bioinformatics.
  5. Demonstrate an understanding of associated molecular biology approaches including recombinant DNA technologies, stem cells and gene therapy.
  6. Obtain, interpret and synthesise information from background reading and use it to effectively supplement lecture notes.
  7. Efficiently and effectively communicate scientific knowledge, experimental results, and analyses, in both oral and written formats.


Assessment details

TypeTask%Timing*Outcomes assessed
Online Quiz Online Mid-Semester Quiz 10% Week 6 1, 6.
Laboratory Report Lab Report 1 (DNA) 15% Week 8 1, 2, 3, 7.
Case Analysis Bioinformatics Case 20% Week 11 4.
Laboratory Report Lab Report 2 (RNA) 15% Week 12 1, 2, 3, 7.
Paper-based Examination (Closed) End of Semester Exam 40% Final Examination Period 1, 2, 3, 4, 5, 6, 7.
  • * Assessment timing is indicative of the week that the assessment is due or begins (where conducted over multiple weeks), and is based on the standard University academic calendar
  • C = Students must reach a level of competency to successfully complete this assessment.

Assessment criteria

High Distinction 85-100 Outstanding or exemplary performance in the following areas: interpretative ability; intellectual initiative in response to questions; mastery of the skills required by the subject, general levels of knowledge and analytic ability or clear thinking.
Distinction 75-84 Usually awarded to students whose performance goes well beyond the minimum requirements set for tasks required in assessment, and who perform well in most of the above areas.
Credit 65-74 Usually awarded to students whose performance is considered to go beyond the minimum requirements for work set for assessment. Assessable work is typically characterised by a strong performance in some of the capacities listed above.
Pass 50-64 Usually awarded to students whose performance meets the requirements set for work provided for assessment.
Fail 0-49 Usually awarded to students whose performance is not considered to meet the minimum requirements set for particular tasks. The fail grade may be a result of insufficient preparation, of inattention to assignment guidelines or lack of academic ability. A frequent cause of failure is lack of attention to subject or assignment guidelines.

Quality assurance

For the purposes of quality assurance, Bond University conducts an evaluation process to measure and document student assessment as evidence of the extent to which program and subject learning outcomes are achieved. Some examples of student work will be retained for potential research and quality auditing purposes only. Any student work used will be treated confidentially and no student grades will be affected.

Study information

Submission procedures

Students must check the [email protected] subject site for detailed assessment information and submission procedures.

Policy on late submission and extensions

A late penalty will be applied to all overdue assessment tasks unless an extension is granted by the subject coordinator. The standard penalty will be 10% of marks awarded to that assessment per day late with no assessment to be accepted seven days after the due date. Where a student is granted an extension, the penalty of 10% per day late starts from the new due date.

Policy on plagiarism

University’s Academic Integrity Policy defines plagiarism as the act of misrepresenting as one’s own original work: another’s ideas, interpretations, words, or creative works; and/or one’s own previous ideas, interpretations, words, or creative work without acknowledging that it was used previously (i.e., self-plagiarism). The University considers the act of plagiarising to be a breach of the Student Conduct Code and, therefore, subject to the Discipline Regulations which provide for a range of penalties including the reduction of marks or grades, fines and suspension from the University.

Bond University utilises Originality Reporting software to inform academic integrity.

Feedback on assessment

Feedback on assessment will be provided to students within two weeks of the assessment submission due date, as per the Assessment Policy.

Disability support

If you have a disability, illness, injury or health condition that impacts your capacity to complete studies, exams or assessment tasks, it is important you let us know your special requirements, early in the semester. Students will need to make an application for support and submit it with recent, comprehensive documentation at an appointment with a Disability Officer. Students with a disability are encouraged to contact the Disability Office at the earliest possible time, to meet staff and learn about the services available to meet your specific needs. Please note that late notification or failure to disclose your disability can be to your disadvantage as the University cannot guarantee support under such circumstances.

Subject curriculum

Central dogma of molecular biology, goals & achievements of the HGP, current & future projects


Methods in nucleic acid isolation, nucleic acid quantitation, electrophoresis


The principles of PCR, modified PCR methods & uses: RT-PCR, quantitative PCR,


The principles of hybridisation, probe detection, hybridisation assays: Southern blotting, molecular cytogenetics, microarrays


Sanger, hierarchical & whole genome approaches, pyrosequencing

3, 4.

Next generation DNA sequencing; $1,000 genome; clinical applications

2, 3, 4.

Biological data, sequence alignments & homology, BLAST, NCBI databases


Molecular diagnosis of inherited diseases, pharmacogenomics, human identification via STRs

2, 3.

Cancer diagnosis, monitoring, prognosis & selection of treatment options

2, 3.

Rapid detection/subtype identification of viruses, bacteria & other infectious pathogens

2, 3.

Molecular typing for HLA- class I & II genes for organ & bone marrow transplants

2, 3.

GM microbes & plants, transgenic mice, stem cells, gene therapy

Approved on: Mar 8, 2019. Edition: 3.4