General Information
In this subject, students will learn about the regulation of gene expression, the human genome and the techniques that are available to study genes. Students will be introduced to the tools of bioinformatics and use these to analyse human genes and genetic diseases. Advanced studies in the molecular biological techniques used to investigate patterns of gene expression will be an important part of this subject. This subject will interest students who wish to undertake a career in a research or a biomedical laboratory setting.
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Details
Academic unit: Faculty of Health Sciences and Medicine Subject code: BMED13-214 Subject title: Molecular Diagnostics Subject level: Undergraduate Semester/Year: May 2024 Credit points: 10.000 -
Delivery & attendance
Timetable: https://bond.edu.au/timetable Delivery mode: Standard Workload items: - Science Lab: x8 (Total hours: 24) - Science Lab
- Forum: x12 (Total hours: 24) - Weekly Forums
- Tutorial: x12 (Total hours: 12) - Weekly Tutorial
- Personal Study Hours: x12 (Total hours: 60) - Personal Study
Attendance and learning activities: Attendance is compulsory Case 1: DNA extraction, PCR-RFLP, electrophoresis, DNA sequencing Case 2: RNA extraction, cDNA synthesis, RT-qPCR, FISH -
Resources
Prescribed resources: No Prescribed resources.
After enrolment, students can check the Books and Tools area in iLearn for the full Resource List.iLearn@Bond & Email: iLearn@Bond is the Learning Management System at Bond University and is used to provide access to subject materials, class recordings and detailed subject information regarding the subject curriculum, assessment, and timing. Both iLearn and the Student Email facility are used to provide important subject notifications.
Additionally, official correspondence from the University will be forwarded to students’ Bond email account and must be monitored by the student.
To access these services, log on to the Student Portal from the Bond University website as www.bond.edu.au
Academic unit: | Faculty of Health Sciences and Medicine |
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Subject code: | BMED13-214 |
Subject title: | Molecular Diagnostics |
Subject level: | Undergraduate |
Semester/Year: | May 2024 |
Credit points: | 10.000 |
Timetable: | https://bond.edu.au/timetable |
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Delivery mode: | Standard |
Workload items: |
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Attendance and learning activities: | Attendance is compulsory Case 1: DNA extraction, PCR-RFLP, electrophoresis, DNA sequencing Case 2: RNA extraction, cDNA synthesis, RT-qPCR, FISH |
Prescribed resources: | No Prescribed resources. After enrolment, students can check the Books and Tools area in iLearn for the full Resource List. |
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iLearn@Bond & Email: | iLearn@Bond is the Learning Management System at Bond University and is used to provide access to subject materials, class recordings and detailed subject information regarding the subject curriculum, assessment, and timing. Both iLearn and the Student Email facility are used to provide important subject notifications. Additionally, official correspondence from the University will be forwarded to students’ Bond email account and must be monitored by the student. To access these services, log on to the Student Portal from the Bond University website as www.bond.edu.au |
Enrolment requirements
Requisites: |
Nil |
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Assumed knowledge: |
Assumed knowledge is the minimum level of knowledge of a subject area that students are assumed to have acquired through previous study. It is the responsibility of students to ensure they meet the assumed knowledge expectations of the subject. Students who do not possess this prior knowledge are strongly recommended against enrolling and do so at their own risk. No concessions will be made for students’ lack of prior knowledge. Assumed Prior Learning (or equivalent):
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Restrictions: |
Nil |
Assurance of learning
Assurance of Learning means that universities take responsibility for creating, monitoring and updating curriculum, teaching and assessment so that students graduate with the knowledge, skills and attributes they need for employability and/or further study.
At Bond University, we carefully develop subject and program outcomes to ensure that student learning in each subject contributes to the whole student experience. Students are encouraged to carefully read and consider subject and program outcomes as combined elements.
Program Learning Outcomes (PLOs)
Program Learning Outcomes provide a broad and measurable set of standards that incorporate a range of knowledge and skills that will be achieved on completion of the program. If you are undertaking this subject as part of a degree program, you should refer to the relevant degree program outcomes and graduate attributes as they relate to this subject.
Subject Learning Outcomes (SLOs)
On successful completion of this subject the learner will be able to:
- Demonstrate knowledge of the techniques that molecular biologists use to manipulate and analyse nucleic acids.
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
- Demonstrate understanding of the Human Genome Project and associated bioinformatics.
- Demonstrate an understanding of associated molecular biology approaches including recombinant DNA technologies, stem cells and gene therapy.
- Obtain, interpret and synthesise information from background reading and use it to effectively supplement lecture notes.
- Efficiently and effectively communicate scientific knowledge, experimental results, and analyses, in both oral and written formats.
Generative Artificial Intelligence in Assessment
The University acknowledges that Generative Artificial Intelligence (Gen-AI) tools are an important facet of contemporary life. Their use in assessment is considered in line with students’ development of the skills and knowledge which demonstrate learning outcomes and underpin study and career success. Instructions on the use of Gen-AI are given for each assessment task; it is your responsibility to adhere to these instructions.
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Assessment details
Type Task % Timing* Outcomes assessed Computer-Aided Examination (Closed) iLearn final- MCQ and SAQ (20% each) 40.00% Final Examination Period 1,2,3,4,5,6,7 Computer-aided Test (Closed) Online iLearn 10.00% Week 6 1,6 Case Analysis Bioinformatics Case 20.00% Week 11 4 Laboratory Report Laboratory report (20%) ad Labster (10%) (4x simulations) 30.00% Ongoing 1,2,3,7 - * Assessment timing is indicative of the week that the assessment is due or begins (where conducted over multiple weeks), and is based on the standard University academic calendar
- C = Students must reach a level of competency to successfully complete this assessment.
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Assessment criteria
Assessment criteria
High Distinction 85-100 Outstanding or exemplary performance in the following areas: interpretative ability; intellectual initiative in response to questions; mastery of the skills required by the subject, general levels of knowledge and analytic ability or clear thinking. Distinction 75-84 Usually awarded to students whose performance goes well beyond the minimum requirements set for tasks required in assessment, and who perform well in most of the above areas. Credit 65-74 Usually awarded to students whose performance is considered to go beyond the minimum requirements for work set for assessment. Assessable work is typically characterised by a strong performance in some of the capacities listed above. Pass 50-64 Usually awarded to students whose performance meets the requirements set for work provided for assessment. Fail 0-49 Usually awarded to students whose performance is not considered to meet the minimum requirements set for particular tasks. The fail grade may be a result of insufficient preparation, of inattention to assignment guidelines or lack of academic ability. A frequent cause of failure is lack of attention to subject or assignment guidelines. Quality assurance
For the purposes of quality assurance, Bond University conducts an evaluation process to measure and document student assessment as evidence of the extent to which program and subject learning outcomes are achieved. Some examples of student work will be retained for potential research and quality auditing purposes only. Any student work used will be treated confidentially and no student grades will be affected.
Type | Task | % | Timing* | Outcomes assessed |
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Computer-Aided Examination (Closed) | iLearn final- MCQ and SAQ (20% each) | 40.00% | Final Examination Period | 1,2,3,4,5,6,7 |
Computer-aided Test (Closed) | Online iLearn | 10.00% | Week 6 | 1,6 |
Case Analysis | Bioinformatics Case | 20.00% | Week 11 | 4 |
Laboratory Report | Laboratory report (20%) ad Labster (10%) (4x simulations) | 30.00% | Ongoing | 1,2,3,7 |
- * Assessment timing is indicative of the week that the assessment is due or begins (where conducted over multiple weeks), and is based on the standard University academic calendar
- C = Students must reach a level of competency to successfully complete this assessment.
Assessment criteria
High Distinction | 85-100 | Outstanding or exemplary performance in the following areas: interpretative ability; intellectual initiative in response to questions; mastery of the skills required by the subject, general levels of knowledge and analytic ability or clear thinking. |
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Distinction | 75-84 | Usually awarded to students whose performance goes well beyond the minimum requirements set for tasks required in assessment, and who perform well in most of the above areas. |
Credit | 65-74 | Usually awarded to students whose performance is considered to go beyond the minimum requirements for work set for assessment. Assessable work is typically characterised by a strong performance in some of the capacities listed above. |
Pass | 50-64 | Usually awarded to students whose performance meets the requirements set for work provided for assessment. |
Fail | 0-49 | Usually awarded to students whose performance is not considered to meet the minimum requirements set for particular tasks. The fail grade may be a result of insufficient preparation, of inattention to assignment guidelines or lack of academic ability. A frequent cause of failure is lack of attention to subject or assignment guidelines. |
Quality assurance
For the purposes of quality assurance, Bond University conducts an evaluation process to measure and document student assessment as evidence of the extent to which program and subject learning outcomes are achieved. Some examples of student work will be retained for potential research and quality auditing purposes only. Any student work used will be treated confidentially and no student grades will be affected.
Study Information
Submission procedures
Students must check the iLearn@Bond subject site for detailed assessment information and submission procedures.
Policy on late submission and extensions
A late penalty will be applied to all overdue assessment tasks unless an extension is granted by the subject coordinator. The standard penalty will be 10% of marks awarded to that assessment per day late with no assessment to be accepted seven days after the due date. Where a student is granted an extension, the penalty of 10% per day late starts from the new due date.
Academic Integrity
Bond University‘s Student Code of Conduct Policy , Student Charter, Academic Integrity Policy and our Graduate Attributes guide expectations regarding student behaviour, their rights and responsibilities. Information on these topics can be found on our Academic Integrity webpage recognising that academic integrity involves demonstrating the principles of integrity (honesty, fairness, trust, professionalism, courage, responsibility, and respect) in words and actions across all aspects of academic endeavour.
Staff are required to report suspected misconduct. This includes all types of plagiarism, cheating, collusion, fabrication or falsification of data/content or other misconduct relating to assessment such as the falsification of medical certificates for assessment extensions. The longer term personal, social and financial consequences of misconduct can be severe, so please ask for help if you are unsure.
If your work is subject to an inquiry, you will be given an opportunity to respond and appropriate support will be provided. Academic work under inquiry will not be marked until the process has concluded. Penalties for misconduct include a warning, reduced grade, a requirement to repeat the assessment, suspension or expulsion from the University.
Feedback on assessment
Feedback on assessment will be provided to students according to the requirements of the Assessment Procedure Schedule A - Assessment Communication Procedure.
Whilst in most cases feedback should be provided within two weeks of the assessment submission due date, the Procedure should be checked if the assessment is linked to others or if the subject is a non-standard (e.g., intensive) subject.
Accessibility and Inclusion Support
Support is available to students where a physical, mental or neurological condition exists that would impact the student’s capacity to complete studies, exams or assessment tasks. For effective support, special requirement needs should be arranged with the University in advance of or at the start of each semester, or, for acute conditions, as soon as practicable after the condition arises. Reasonable adjustments are not guaranteed where applications are submitted late in the semester (for example, when lodged just prior to critical assessment and examination dates).
As outlined in the Accessibility and Inclusion Policy, to qualify for support, students must meet certain criteria. Students are also required to meet with the Accessibility and Inclusion Advisor who will ensure that reasonable adjustments are afforded to qualifying students.
For more information and to apply online, visit BondAbility.
Additional subject information
Subject curriculum
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1. The Human Genome Project
Central dogma of molecular biology, goals & achievements of the HGP, current & future projects.
SLOs included
- Demonstrate understanding of the Human Genome Project and associated bioinformatics.
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2. DNA/RNA Isolation, Visualization & Quantitatio
Methods in nucleic acid isolation, nucleic acid quantitation, electrophoresis.
SLOs included
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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3. Polymerase Chain Reaction (PCR)
The principles of PCR, modified PCR methods & uses: RT-PCR, quantitative PCR.
SLOs included
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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4. Nucleic Acid Hybridisation
The principles of hybridisation, probe detection, hybridisation assays: Southern blotting, molecular cytogenetics, microarrays.
SLOs included
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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5. DNA Sequencing
Sanger, hierarchical & whole genome approaches, pyrosequencing.
SLOs included
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
- Demonstrate understanding of the Human Genome Project and associated bioinformatics.
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6. Next Generation DNA Sequencing
Next generation DNA sequencing; $1,000 genome; clinical applications.
SLOs included
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
- Demonstrate understanding of the Human Genome Project and associated bioinformatics.
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7. Bioinformatics
Biological data, sequence alignments & homology, BLAST, NCBI databases.
SLOs included
- Demonstrate understanding of the Human Genome Project and associated bioinformatics.
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8. Inherited Diseases/Pharmacogenomics/Human ID
Molecular diagnosis of inherited diseases, pharmacogenomics, human identification via STRs.
SLOs included
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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9. Molecular Oncology
Cancer diagnosis, monitoring, prognosis & selection of treatment options.
SLOs included
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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10. Detection & Identification of Microbes
Rapid detection/subtype identification of viruses, bacteria & other infectious pathogens.
SLOs included
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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11. Tissue Typing
Molecular typing for HLA- class I & II genes for organ & bone marrow transplants.
SLOs included
- Demonstrate knowledge of how molecular biology is used in diagnostic laboratories for the detection and monitoring of disease.
- Carry out DNA/RNA isolation and analysis methods including PCR, genotyping by RFLP, DNA sequencing, RT-qPCR and FISH
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12. Beyond Molecular Diagnostics
GM microbes & plants, transgenic mice, stem cells, gene therapy.
SLOs included
- Demonstrate an understanding of associated molecular biology approaches including recombinant DNA technologies, stem cells and gene therapy.